Prueba rápida de IgG/IgG/IgM de MonkeoPox
Componentes del kit
• Dispositivos de prueba
• Goteros
• Buffer único
• Inserto de paquete
Principio
The Monkeypox virus IgG/IgM Rapid Test is a qualitative membrane-based immunoassay for the detection of Monkeypox antibodies in whole blood, serum, or plasma. This test consists of two components, an IgG component and an IgM component. In the IgG component, anti-human IgG is coated in test line region G of the test. During testing, the specimen reacts with Monkeypox antigen-coated particles in the test strip. The mixture then migrates upward on the membrane chromatographically by capillary action and reacts with the anti-human IgG in test line region G. If the specimen contains IgG antibodies to Monkeypox, a colored line will appear in test line region G. In the IgM component, anti-ligand is coated in test line region M of the test. During testing, the specimen reacts with ligand anti-human IgM. Monkeypox IgM antibodies, if present in the specimen, reacts with the ligand anti-human IgM and the Monkeypox antigen-coated particles in the test strip, and this complex is captured by the anti-ligand, forming a colored line in test line region M. Therefore, if the specimen contains Monkeypox IgG antibodies, a colored line will appear in test line region G. If the specimen contains Monkeypox IgM antibodies, a colored line will appear in test line region M. If the specimen does not contain Monkeypox antibodies, no colored line will appear in either of the test line regions, indicating a negative result. To serve as a procedural control, a colored line will always change from blue to red in the control line region, indicating that theproper volume of specimen has been added and membrane wicking has occurred.
Procedimiento
Allow the test device, specimen, buffer, and/or controls to reach room Temperatura (15 - 30 ° C) antes de la prueba.
1.Bring the pouch to room temperature before opening. Remove the test Dispositivo de la bolsa sellada y úsela lo antes posible.
2.Place the test device on a clean and level surface. For Serum or Plasma Specimens: Hold the dropper vertically, draw the specimen up to the Fill Line (aproximadamente 10 ul) y transfiera el espécimen al
specimen well (S) of the test device, then add 2 drops of buffer (approximately 80 mL) and start the timer. See illustration below. Avoid trapping air bubbles in the Muestra (s) muestra (s).
For Whole Blood (Venipuncture/Fingerstick) Specimens: To use a dropper: Hold the dropper vertically, draw the specimen 0.5-1 cm above the Fill Line, and transfer 2 drops of whole blood (approximately 20 µL) to the specimen well (S) of the test device, then add 2 drops of Buffer (aproximadamente 80 UL) e inicie el temporizador. Ver ilustración a continuación.To use a micropipette: Pipette and dispense 20 µL of whole blood to the specimen well (S) of the test device, then add 2 drops of buffer (aproximadamente 80 µl) e inicie el temporizador.
3.Wait for the colored line(s) to appear. Read results at 10 minutes. Do not Interpreta el resultado después de 20 minutos.
Interpretación del resultado del ensayo
