Monkeypox -Virus IgG/IgM -Schnelltest
Kit -Komponenten
• Testgeräte
• Tropfen
• Einzelpuffer
• Paketeinsatz
Prinzip
The Monkeypox virus IgG/IgM Rapid Test is a qualitative membrane-based immunoassay for the detection of Monkeypox antibodies in whole blood, serum, or plasma. This test consists of two components, an IgG component and an IgM component. In the IgG component, anti-human IgG is coated in test line region G of the test. During testing, the specimen reacts with Monkeypox antigen-coated particles in the test strip. The mixture then migrates upward on the membrane chromatographically by capillary action and reacts with the anti-human IgG in test line region G. If the specimen contains IgG antibodies to Monkeypox, a colored line will appear in test line region G. In the IgM component, anti-ligand is coated in test line region M of the test. During testing, the specimen reacts with ligand anti-human IgM. Monkeypox IgM antibodies, if present in the specimen, reacts with the ligand anti-human IgM and the Monkeypox antigen-coated particles in the test strip, and this complex is captured by the anti-ligand, forming a colored line in test line region M. Therefore, if the specimen contains Monkeypox IgG antibodies, a colored line will appear in test line region G. If the specimen contains Monkeypox IgM antibodies, a colored line will appear in test line region M. If the specimen does not contain Monkeypox antibodies, no colored line will appear in either of the test line regions, indicating a negative result. To serve as a procedural control, a colored line will always change from blue to red in the control line region, indicating that theproper volume of specimen has been added and membrane wicking has occurred.
Verfahren
Allow the test device, specimen, buffer, and/or controls to reach room Temperatur (15 - 30 ° C) Vor dem Test.
1.Bring the pouch to room temperature before opening. Remove the test Gerät aus dem versiegelten Beutel und verwenden Sie ihn so schnell wie möglich.
2.Place the test device on a clean and level surface. For Serum or Plasma Specimens: Hold the dropper vertically, draw the specimen up to the Fill Line (ungefähr 10 ul) und das Probe an die
specimen well (S) of the test device, then add 2 drops of buffer (approximately 80 mL) and start the timer. See illustration below. Avoid trapping air bubbles in the Exemplarbrunnen (en).
For Whole Blood (Venipuncture/Fingerstick) Specimens: To use a dropper: Hold the dropper vertically, draw the specimen 0.5-1 cm above the Fill Line, and transfer 2 drops of whole blood (approximately 20 µL) to the specimen well (S) of the test device, then add 2 drops of Puffer (ca. 80 UL) und den Timer starten. Siehe Abbildung unten.To use a micropipette: Pipette and dispense 20 µL of whole blood to the specimen well (S) of the test device, then add 2 drops of buffer (ungefähr 80 µl) und den Timer starten.
3.Wait for the colored line(s) to appear. Read results at 10 minutes. Do not Interpretieren Sie das Ergebnis nach 20 Minuten.
Interpretation des Assay -Ergebniss
