Norovírus (Gⅰ/Gⅱ) Dispositivo de teste rápido (fezes)
Princípio
The Norovirus (GⅠ/GⅡ) Rapid Test Device (Feces) has been designed to detect norovirus GⅠ and GⅡ through visual interpretation of color development in the internal strip. The membrane was immobilized with anti-norovirus GⅠ antibodies and anti-norovirus GⅡ antibodies on the test region. During the test, the specimen is allowed to react with colored anti-norovirus GⅠ antibodies colloidal gold conjugates and anti norovirus GⅡ antibodies colloidal gold conjugates, which were precoated on the sample pad of the test. The mixture then moves on the membrane by a capillary action, and interact with reagents on the membrane. If there were enough norovirus GⅠ in specimens, a colored band will form at the T1 region of the membrane.
Da mesma forma, se houvesse norovírus Gⅱ suficientes em espécimes, uma banda colorida se formará na região T2 da membrana. A presença de bandas coloridas indica um resultado positivo, enquanto sua ausência indica um resultado negativo. A aparência de uma banda colorida na região de controle serve como um controle processual. Isso indica que o volume adequado de amostra foi adicionado e ocorreu o wicking da membrana.
Direções para uso
Traga testes, amostras, tampão e/ou controles para a temperatura ambiente (15 - 30 ° C) antes do uso.
1. Coleta de amostras e pré -tratamento:
1) Use o contêiner de coleta de amostras para coleção de amostras. Os melhores resultados serão obtidos se o ensaio for realizado dentro de 6 horas após a coleta.
2) For solid specimens: Unscrew and remove the dilution tube applicator. Be careful not to spill or spatter solution from the tube. Collect specimens by inserting the applicator stick into at least 3 different sites of the feces to collect approximately 125 mg of feces .
For liquid specimens: Hold the pipette vertically, aspirate fecal specimens, and then transfer 125 µL into the specimen collection tube containing the extraction buffer.
3) Place the applicator back into the tube and screw the cap tightly. Be careful not to break the tip of the dilution tube.
4) Agite o tubo de coleta de amostras vigorosamente para misturar a amostra e o buffer de extração.
Specimens prepared in the specimen collection tube may be stored for 6 months at -20°C if not tested within 1 hour after preparation.
2. Teste
1) Remove the test from its sealed pouch, and place it on a clean, level surface. Label the test with patient or control identification. To obtain a best result, the assay should be performed within one hour.
2) Using a piece of tissue paper, break the tip of the dilution tube. Hold the tube vertically and dispense 4 drops of solution into the specimen well (S) of the test device.
Avoid trapping air bubbles in the specimen well (S), and do not drop any solution in observation window.
À medida que o teste começa a funcionar, você verá a cor se mover através da membrana.
3. Aguarde que as bandas coloridas apareçam. O resultado deve ser lido em 10 minutos.
