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Ngwaahịa

Ngwa ule Chlamydia ngwa ngwa


Nkọwa dị mkpirikpi:

Ngwa ule Chlamydia ngwa ngwa bụ immunoassay chromatographic ngwa ngwa maka nchọpụta qualitative nke Chlamydia trachomatis n'ụdị ụlọ ọgwụ iji nyere aka n'ịchọpụta ọrịa Chlamydia.



Nkọwa ngwaahịa

Mkpado ngwaahịa

Ụkpụrụ

Ọ bụ qualitative, mpụta eruba immunoassay maka nchọpụta nke Chlamydia antigen si n'ụdị ụlọ ọgwụ. N'ime ule a, a na-ekpuchi ọgwụ mgbochi kpọmkwem na Chlamydia antigen na mpaghara akara ule nke warara. N'oge a na-anwale ule, ihe ngwọta antigen ewepụtara na-emeghachi omume na mgbochi nke Chlamydia nke a na-ekpuchi n'ime ụmụ irighiri ihe. Ngwakọta ahụ na-akwaga elu iji meghachi omume na mgbochi Chlamydia na akpụkpọ ahụ wee mepụta ahịrị uhie na mpaghara ule.

Mkpachapụ anya

Biko gụọ ozi niile dị na ntinye ngwugwu a tupu ịme ule ahụ.

● Maka ọkachamara in vitro diagnostic were naanị. Ejila ụbọchị ngafe gachara.
● Aṅụla ihe ọṅụṅụ, ma ọ bụ sịga n'ebe a na-edozi ihe nlere na ngwa.
● Dezie ihe nlere anya dị ka à ga-asị na ha nwere ihe ndị na-efe efe. Leba anya n'akpachapụ anya siri ike megide ihe egwu microbiological n'ime usoro a niile wee soro usoro ọkọlọtọ maka mkpofu nke ọma.
● Yiri uwe nchebe dị ka uwe nyocha ụlọ nyocha, uwe mkpuchi a na-atụfu ya na nchebe anya mgbe a na-enyocha ihe nlele.
● Iru mmiri na okpomọkụ nwere ike imetụta nsonaazụ.
● Jiri naanị swabs na-adịghị mma iji nweta ihe nlere anya endocervical.
● Mbadamba ụrọ nke tindazole na-emepụta ihe na Confort Pessaries nwere ụdị adịghị mma nwere ike ime ka nnyonye anya adịghị ike.

Ntuziaka maka ojiji

Kwe ka ngwaọrụ nwale, ụdị, reagents na/ma ọ bụ njikwa ka ọ ruo n'ime ụlọ (15-30 C) tupu nnwale.

1. Wepu ngwaọrụ ule na obere akpa foil mechiri emechi ma jiri ya ozugbo enwere ike. A ga-enweta nsonaazụ kacha mma ma ọ bụrụ na a na-eme ule ahụ ozugbo emepechara obere akpa foil.

2. Wepụ Chlamydia antigen:
Maka Ụdị Cervical nwanyị ma ọ bụ nwoke Urethral Swab:
Jide karama reagent ahụ kwụ ọtọ ma tinye 4 zuru ezu nke Reagent A (ihe dị ka 280µL) na tube mmịpụta (Lee ihe atụ ①). Reagent A enweghị agba. Fanye swab ozugbo, kpachie ala nke tube wee tụgharịa swab ugboro iri na ise. Hapụ maka nkeji 2. (Lee ihe atụ ②)

Jide karama Reagent B kwụ ọtọ ma tinye 4 zuru ezu Reagent B (ihe dị ka 240ul) na tube mmịpụta. (Lee ihe atụ ③) Reagent B na-acha odo odo. Ihe ngwọta ga-atụgharị urukpuru. Kwakọba ala nke tube ma tụgharịa swab ugboro 15 ruo mgbe ngwọta ahụ ghọrọ agba doro anya nke nwere ntakịrị tint green ma ọ bụ anụnụ anụnụ. Ọ bụrụ na swab ahụ na-agba ọbara, agba ga-agbanwe edo edo ma ọ bụ aja aja. Hapụ maka nkeji 1. (Lee ihe atụ ④)

Pịa swab n'akụkụ akụkụ nke tube ma wepụ swab mgbe ị na-amanye tube ahụ. (Lee ihe atụ ⑤)) Debe oke mmiri n'ime tube dịka o kwere mee. Dakọta ọnụ dropper n'elu tube mmịpụta. (Lee ihe atụ ⑥)

Maka ụdị mmamịrị nwoke:
Jide karama Reagent B kwụ ọtọ wee tinye 4 zuru ezu Reagent B (ihe dị ka 240ul) na pellet mmamịrị dị na tube centrifuge, wee maa jijiji nke ukwuu ruo mgbe nkwusioru ahụ ga-adakọ.

Nyefee ihe ngwọta niile na tube centrifuge na tube mmịpụta. Hapụ maka nkeji 1.

Jide karama Reagent A kwụ ọtọ wee gbakwunye mkpọda Reagent A 4 zuru oke (ihe dịka 280 µL) wee tinye na tube mmịpụta. Vortex ma ọ bụ kpatụ ala nke tube iji gwakọta ihe ngwọta. Ka guzoro nkeji 2.

Dakọta ọnụ dropper n'elu tube mmịpụta.
3. Debe ngwaọrụ ule na elu dị ọcha na larịị. Tinye ntụzịaka 3 zuru ezu nke ngwọta ewepụtara (ihe dị ka 100 µL) n'ụdị nke ọma (S) nke ngwaọrụ nnwale, wee malite ngụ oge. Zenarị ọnyà ikuku n'ime olulu mmiri (S).

4. Chere ka ahịrị uhie pụtara. Gụọ nsonaazụ ya na nkeji iri. Agụla nsonaazụ mgbe nkeji iri abụọ gachara.

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EZIOKWU:
* Otu agba nwere agba na-apụta na mpaghara njikwa njikwa (C) yana otu agba agba ọzọ na-apụta na mpaghara T band.

Rịzọlt na-adịghị mma:
Otu eriri nwere agba na-egosi na mpaghara njikwa njikwa (C). Ọnweghị eriri apụta na mpaghara band band (T).

Nsonaazụ ezighi ezi:
Otu njikwa adaghị apụta. A ga-atụfurịrị nsonaazụ sitere na ule ọ bụla nke na-emepụtabeghị igwe njikwa n'oge agụpụtara akọwapụtara. Biko lelee usoro a wee jiri ule ọhụrụ kwugharịa. Ọ bụrụ na nsogbu ahụ dịgidere, kwụsị iji ngwa ngwa ngwa wee kpọtụrụ onye nkesa mpaghara gị.
* IHE: Ike nke agba uhie na mpaghara akara ule (T) nwere ike ịdịgasị iche dabere na mkpokọta Chlamydia antigen na-egosi n'ụdị. Ya mere, ndò ọ bụla nke na-acha uhie uhie na mpaghara ule (T) kwesịrị ka a na-ewere ya dị mma.


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